Isolation of 1-aminocyclopropane-1-carboxylate synthase gene from Oncidium Gower Ramsey
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چکیده
منابع مشابه
Isolation of 1-aminocyclopropane-1-carboxylate synthase gene from Oncidium Gower Ramsey.
A full-length cDNA of a 1-aminocyclopropane-1-carboxylate synthase (ACS) family member from Oncidium, named OnACS1 (GenBank accession No. JQ822087) was cloned and characterized by reverse transcription polymerase chain reaction and rapid amplification of cDNA ends technology. The full-length cDNA was 1586 bp, including a 1308-bp open reading frame, a 105-bp 5' untranslated region (UTR), and 173...
متن کاملIsolation, Cloning and Sequence Analysis of 1-Aminocyclopropane-1-Carboxylate Deaminase Gene from Native Sinorhizobium meliloti
Background: Many plant growth-promoting bacteria including Rhizobia contain the 1-aminocyclopropane-1-carboxylate (ACC) deaminase enzyme that can leave ACC, and thereby lower the level of ethylene in stressed plants. Drought and salinity are the most common environmental stress factors for plants in Iran. Objectives: The main aim of this research was development of bio-fertilizers containing A...
متن کاملisolation, cloning and sequence analysis of 1-aminocyclopropane-1-carboxylate deaminase gene from native sinorhizobium meliloti
background: many plant growth-promoting bacteria including rhizobia contain the 1-aminocyclopropane-1-carboxylate (acc) deaminase enzyme that can leave acc, and thereby lower the level of ethylene in stressed plants. drought and salinity are the most common environmental stress factors for plants in iran. objectives: the main aim of this research was development of bio-fertilizers containing ac...
متن کاملProperties and Partial Purification of 1-Aminocyclopropane-1-carboxylate Synthase.
We studied the regulation of 1-aminocyclopropane-1-carboxylate (ACC) synthase activity in tomato (Lycopersicon esculentum Mill.) fruit tissue and attempted the purification of this enzyme. The increase of ACC synthase activity in wounded tomato pericarp was inhibited by cordycepin and cycloheximide. Density labeling studies showed a 0.75% increase in the buoyant density of ACC synthase isolated...
متن کاملThe 1-aminocyclopropane-1-carboxylate synthase gene family of Arabidopsis thaliana.
Genomic sequences encoding five divergent 1-aminocyclopropane-1-carboxylic acid (ACC) synthase polypeptides (ACC1, ACC2, ACC3, ACC4, and ACC5) have been isolated from Arabidopsis thaliana by using heterologous cDNAs and PCR fragments amplified from genomic DNA with degenerate oligonucleotide primers. Each gene is located on a different chromosome in the Arabidopsis genome. The genes are differe...
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ژورنال
عنوان ژورنال: Genetics and Molecular Research
سال: 2014
ISSN: 1676-5680
DOI: 10.4238/2014.october.20.24